Development of a sensitive polymerase chain reaction (PCR) and digoxigenin (DIG)-labeled in situ hybridization (ISH) for the detection of Vibrio bacteria in the Pacific oyster Crassostrea gigas
Vibrio bacteria are one of the greatest threats to aquaculture, with Vibrio-associated mass mortality events occurring in all life stages worldwide.Vibrio aestuarianus is particularly problematic in Pacific oyster Crassostrea gigas cultures.A diagnostic method used to detect V.aestuarianus is quantitative (q)PCR Fridge Roller Base and a confirmator